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Methadone is a synthetic, long-acting opioid with pharmacologic actions qualitatively similar to morphine and is active by oral and parenteral routes of administration. It is primarily a μ-receptor agonist and may mimic endogenous opioids, enkephalins, and endorphins and affect the release of other neurotransmitters acetylcholine, norepinephrine, substance P, and dopamine.

Methadone 40mg works to treat pain by changing the way the brain and nervous system respond to pain. It works to treat people who were addicted to opiate drugs by producing similar effects and preventing withdrawal symptoms in people who have stopped using these drugs.